Gold Nanoparticles Treatment Reverses Brain Damage in Alzheimer’s Disease Model
https://link.springer.com/article/10.1007/s12035-019-01780-w
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Molecular Neurobiology volume 57, pages926–936(2020)Cite this article
- Natalia dos Santos Tramontin,
- Sabrina da Silva,
- Rychard Arruda,
- Kellen Simon Ugioni,
- Paula Bortuluzzi Canteiro,
- Gustavo de Bem Silveira,
- Carolini Mendes,
- Paulo Cesar Lock Silveira &
- Alexandre Pastoris Muller
Abstract
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Alzheimer’s disease (AD) is characterized by amyloid (A)β peptide accumulation and intracellular neurofibrillary tangles. New hypotheses have suggested that AD involves neuroinflammation and oxidative stress. Gold nanoparticles (AuNP) presents anti-inflammatory and antioxidant characteristics. The present study evaluated the AuNP treatment on an AD model (okadaic acid, OA). Male Wistar rats were injected intracerebroventricularly with OA (100 μg); 24 h later they were treated with 20-nm AuNP (at a dose 2.5 mg/kg) every 48 h for 21 days. The following groups were separated (n = 12/group): Sham, AuNP, OA, and OA + AuNP. OA increases Tau phosphorylation in the cortex and hippocampus, while AuNP treatment maintained it as normal. Spatial memory was impaired by OA, and AuNP treatment prevented this deficit. Neurotrophic factors (BDNF and NGF- β) in the cortex and hippocampus were decreased by OA. The OA and OA + AuNP groups showed increased interleukin (IL)-1 β in the hippocampus and cortex, and the AuNP group showed increased IL-1 β in the hippocampus. In both groups, S100 levels in the cortex and hippocampus were increased by OA. IL-4 was increased in OA + AuNP animals. AuNPs prevented oxidative stress (sulfhydryl and nitrite levels) in brain structures induced by OA. Moreover, OA modulated ATP synthase activity, and AuNP maintained normal brain mitochondrial function. The antioxidant capacities were reduced by OA, and AuNP restored antioxidant status (SOD, catalase activities and GSH levels) on brain. OA-induced damage on brain tissues, and long-term AuNP treatment prevented the neuroinflammation, modulation of mitochondrial function, and impaired cognition induced by AD model, showing that AuNPs may be a promising treatment for neurodisease caused by these elements.
요약
알츠하이머 병 (AD)은 아밀로이드 (A) β 펩티드 축적 및 세포 내 신경 섬유 엉킴을 특징으로 한다. 새로운 가설은 AD가 신경 염증 및 산화적 스트레스를 수반한다고 제안했다. 금 나노 입자 (AuNP)는 항염증 및 항산화 특성을 나타냅니다. 본 연구는 AD모델 (오카다산, OA)에 대한 AuNP 처리를 평가 하였다. 수컷 Wistar 랫트에 뇌 실내 OA (100 μg)를 주사 하였다; 24 시간 후, 21 일 동안 48 시간마다 20nm AuNP (용량 2.5 mg / kg)로 처리 하였다. Sham, AuNP, OA 및 OA + AuNP 그룹은 분리되었다 (n == 12 / 그룹). ONP 처리는 피질과 해마에서 타우 인산화를 증가시키는 반면, ONP 처리는이를 정상적으로 유지 하였다. 공간 기억은 OA에 의해 손상되었고, AuNP 처리는 이러한 결손을 방지했다. 피질과 해마의 신경 영양 인자 (BDNF와 NGF-β)는 OA에 의해 감소되었다. OA와 OA + AuNP 군은 해마와 피질에서 인터루킨 (IL) -1β가 증가한 것으로 나타 났으며, AuNP 군은 해마에서 IL-1β가 증가한 것으로 나타났다. 두 그룹에서, 피질 및 해마의 S100 수준은 OA에 의해 증가되었다. IL-4는 OA + AuNP 동물에서 증가했다. AuNP는 OA에 의해 유도 된 뇌 구조에서 산화 스트레스 (설프 하이 드릴 및 아질산염 수준)를 방지 하였다. 또한, OA는 ATP 신타 제 활성을 조절하였고, AuNP는 정상적인 뇌 미토콘드리아 기능을 유지 하였다. 산화 방지제 용량은 OA에 의해 감소되었고, AuNP는 뇌에서 산화 방지제 상태 (SOD, 카탈라제 활성 및 GSH 수준)를 회복시켰다. 뇌 조직에 OA- 유도 된 손상 및 장기 AuNP 처리는 신경 염증, 미토콘드리아 기능의 조절 및 AD 모델에 의해 유발 된인지 장애를 예방하여, AuNP가 이들 요소에 의해 야기되는 신경 질환에 대한 유망한 치료 일 수 있음을 보여 주었다.
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Funding
This work was supported by FAPESC-PPSUS 2016, CNPq, Instituto Nacional de Neurociência Translacional-INNT #465346/2014-6, and Unesc.
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- Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, Bairro Universitário, Criciúma, SC, 88806-000, Brazil
- Natalia dos Santos Tramontin
- , Sabrina da Silva
- , Kellen Simon Ugioni
- , Paula Bortuluzzi Canteiro
- , Gustavo de Bem Silveira
- , Carolini Mendes
- , Paulo Cesar Lock Silveira
- & Alexandre Pastoris Muller
- Universidade de Rio Verde, Rio Verde, GO, 75901-970, Brazil
- Rychard Arruda
- Programa de Pos Graduação em Farmacologia, UFSC, Florianópolis, SC, Brazil
- Alexandre Pastoris Muller
Correspondence to Alexandre Pastoris Muller.
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우리는 은銀용액 Colloidal Silver 또는 금 金용액 Colloidal Gold을 의약품이라 주장하지 않습니다. 이곳에서는 나노 은 (AgNPs) 및 나노 금(AuNPs)입자와 이온의 효능과 작용에 대해 연구 조사하고 정보를 제공합니다.Dismiss Notice
은용액이나 금용액을 건강 목적으로 사용하는 경우 사용자는 충분한 지식과 이해를 바탕으로 자신의 책임을 주장할 수 있어야 합니다. -
이 곳에서 인용되거나 연결된 문서 및 정보는 특별한 명시가 없는 한 공개와 무료로 사용이 가능하다는 관례에 따르며, 해당 문서 및 정보의 배포자가 저작권을 주장하는 범위에 따라 저작 및 소유권은 해당 권리자에게 있습니다. 이 곳에 공개된 다른 문서는 우리 사이트에 등록된 것임을 명시하는 것을 전제로 수정하지 않고 배포, 저장 등 자유롭게 사용할 수 있습니다.
금 나노 금 나노 입자는 알츠하이머 질병 모델에서 뇌손상을 역전
Discussion in '금Gold 은silver 요법' started by Mind Central, 2020-03-07 02:17.
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by Mind Central, 2020-03-07 02:17 at 2:17 AM
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